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lukas kapitein  (Addgene inc)


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    Structured Review

    Addgene inc lukas kapitein
    Lukas Kapitein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lukas+kapitein/pE4c+(Plasmid+%2317464)/bio_rxiv__2025__08__05__668525-205-18-20
    Average 93 stars, based on 3 article reviews
    lukas kapitein - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Knock-Out:

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: The following secondary antibodies were used for (IB): goat anti-mouse IgG(H+L) IRDye 680LT (926-68020, LI-COR), goat anti-rabbit IgG(H+L) IRDye800CW (926-32211, LI-COR); secondary antibodies (IF): goat anti-rabbit IgG(H+L) Alexa Fluor Plus 647 (A32733, Invitrogen), goat anti-mouse IgG(H+L) Alexa Fluor Plus 647 (A32728, Invitrogen). .. The Brunello knockout pooled library was a gift from David Root and John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSpCas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mitoBFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGEmt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request. .. The chemicals and reagents used in this study include: 2-Deoxy-Dglucose (D8375-1G, Sigma), 2-mercaptoethanol (BME) (M6250100ML, Sigma), agar (A10752, AlphaAesar), agarose (16500500, Thermo Fisher), ampicillin (A9518-25G, Sigma), Bafilomycin-A1 (11038, Caymen Chemical), Beta-glycerophosphate (35675-50GM, Sigma), blasticidin (ant-bl-1, Invivogen), Bortezomib (10008822, Caymen Chemical), Brefeldin-A1 (11861, Caymen Chemical), CB5083 (19311, Caymen Chemical), dimethyl sulfoxide (C833V25, Thomas Scientific), EDTA (EDS-500G, Sigma), glycerol (G20251L, Sigma), HEPES (H3375-1KG, Sigma), hygromycin (ant-hg-1, Invivogen), kanamycin (BP906-5, FisherSci), MLN-4924 (15217, Caymen Chemical), MLN-7243 (30108, Caymen Chemical), plasmocin (ant-mpp, Invivogen), Phusion High-Fidelity DNA polymerase (M0530L, NEB), PIK-III (17002, Caymen Chemical), polybrene (H9268-5G, Sigma), potassium chloride (P217-500, FisherSci), puromycin (ant-pr-1, Invivogen), sodium chloride (6438, FisherSci), sodium deoxycholate (97062-028, VWR), sodium dodecyl sulfate (SDS) (74255-250G, Sigma), sodium fluoride (S6776-100G, Sigma), sodium orthovanadate (450243- 10G, Sigma), sodium pyrophosphate decahydrate (221368-100G, Sigma), Taq DNA ligase (M0208L, NEB), TERGITOL Type NP-40 (NP40S-100ML, Sigma), Tris base (T1378-5KG, Sigma), Triton X-100 (T9284-500ML, Sigma), tryptone (DF0123-17-3, FisherSci), Tween-20 (BP337-500, FisherSci), T5 exonuclease (M0363S, NEB), yeast extract (BP1422-2, FisherSci), and zeocin (ant-zn-1, Invivogen).

    Plasmid Preparation:

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: The following secondary antibodies were used for (IB): goat anti-mouse IgG(H+L) IRDye 680LT (926-68020, LI-COR), goat anti-rabbit IgG(H+L) IRDye800CW (926-32211, LI-COR); secondary antibodies (IF): goat anti-rabbit IgG(H+L) Alexa Fluor Plus 647 (A32733, Invitrogen), goat anti-mouse IgG(H+L) Alexa Fluor Plus 647 (A32728, Invitrogen). .. The Brunello knockout pooled library was a gift from David Root and John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSpCas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mitoBFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGEmt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request. .. The chemicals and reagents used in this study include: 2-Deoxy-Dglucose (D8375-1G, Sigma), 2-mercaptoethanol (BME) (M6250100ML, Sigma), agar (A10752, AlphaAesar), agarose (16500500, Thermo Fisher), ampicillin (A9518-25G, Sigma), Bafilomycin-A1 (11038, Caymen Chemical), Beta-glycerophosphate (35675-50GM, Sigma), blasticidin (ant-bl-1, Invivogen), Bortezomib (10008822, Caymen Chemical), Brefeldin-A1 (11861, Caymen Chemical), CB5083 (19311, Caymen Chemical), dimethyl sulfoxide (C833V25, Thomas Scientific), EDTA (EDS-500G, Sigma), glycerol (G20251L, Sigma), HEPES (H3375-1KG, Sigma), hygromycin (ant-hg-1, Invivogen), kanamycin (BP906-5, FisherSci), MLN-4924 (15217, Caymen Chemical), MLN-7243 (30108, Caymen Chemical), plasmocin (ant-mpp, Invivogen), Phusion High-Fidelity DNA polymerase (M0530L, NEB), PIK-III (17002, Caymen Chemical), polybrene (H9268-5G, Sigma), potassium chloride (P217-500, FisherSci), puromycin (ant-pr-1, Invivogen), sodium chloride (6438, FisherSci), sodium deoxycholate (97062-028, VWR), sodium dodecyl sulfate (SDS) (74255-250G, Sigma), sodium fluoride (S6776-100G, Sigma), sodium orthovanadate (450243- 10G, Sigma), sodium pyrophosphate decahydrate (221368-100G, Sigma), Taq DNA ligase (M0208L, NEB), TERGITOL Type NP-40 (NP40S-100ML, Sigma), Tris base (T1378-5KG, Sigma), Triton X-100 (T9284-500ML, Sigma), tryptone (DF0123-17-3, FisherSci), Tween-20 (BP337-500, FisherSci), T5 exonuclease (M0363S, NEB), yeast extract (BP1422-2, FisherSci), and zeocin (ant-zn-1, Invivogen).

    Article Title: The Rab5 effector Rabankyrin-5 mediates endosomal fusion and trafficking of Human Papillomavirus during early entry
    Article Snippet: The plasmids mRFP-Rab5 and FLAG-Rab5A were gifts from Drs. Ari Helenius (Addgene plasmid #14437) and Qing Zhong (Addgene plasmid #28043), respectively. .. For the peroxisome relocation assay, pBa.PEX3-tdTomato-FKBP and HA-BICD2(1–500)-FRB were gifts from Drs. Marvin Bentley (Addgene plasmid #193941) and Lukas Kapitein (Addgene plasmid #174648). ..

    Generated:

    Article Title: The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover.
    Article Snippet: The following secondary antibodies were used for (IB): goat anti-mouse IgG(H+L) IRDye 680LT (926-68020, LI-COR), goat anti-rabbit IgG(H+L) IRDye800CW (926-32211, LI-COR); secondary antibodies (IF): goat anti-rabbit IgG(H+L) Alexa Fluor Plus 647 (A32733, Invitrogen), goat anti-mouse IgG(H+L) Alexa Fluor Plus 647 (A32728, Invitrogen). .. The Brunello knockout pooled library was a gift from David Root and John Doench (Addgene #73178). psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260). pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid #8454). lentiCRISPRv2puro was a gift from Brett Stringer (Addgene plasmid #98290). lentiGuide-puro was a gift from Feng Zhang (Addgene plasmid #52963). pFUGW-EFSpCas9-P2A-Zeo (pAWp30) was a gift from Timothy Lu (Addgene plasmid #73857). pLenti CMV GFP Puro (658-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid #17448). mitoBFP was a gift from Gia Voeltz (Addgene # 49151). pGW1-mCherry-EGFP-PIM was a gift from Lukas Kapitein (Addgene plasmid #111759). pHAGEmt-mKeima was a gift from Richard Youle (Addgene plasmid #131626). pLKO.1 hygro was a gift from Bob Weinberg (Addgene plasmid # 24150) Other vectors generated during this study are available upon request. .. The chemicals and reagents used in this study include: 2-Deoxy-Dglucose (D8375-1G, Sigma), 2-mercaptoethanol (BME) (M6250100ML, Sigma), agar (A10752, AlphaAesar), agarose (16500500, Thermo Fisher), ampicillin (A9518-25G, Sigma), Bafilomycin-A1 (11038, Caymen Chemical), Beta-glycerophosphate (35675-50GM, Sigma), blasticidin (ant-bl-1, Invivogen), Bortezomib (10008822, Caymen Chemical), Brefeldin-A1 (11861, Caymen Chemical), CB5083 (19311, Caymen Chemical), dimethyl sulfoxide (C833V25, Thomas Scientific), EDTA (EDS-500G, Sigma), glycerol (G20251L, Sigma), HEPES (H3375-1KG, Sigma), hygromycin (ant-hg-1, Invivogen), kanamycin (BP906-5, FisherSci), MLN-4924 (15217, Caymen Chemical), MLN-7243 (30108, Caymen Chemical), plasmocin (ant-mpp, Invivogen), Phusion High-Fidelity DNA polymerase (M0530L, NEB), PIK-III (17002, Caymen Chemical), polybrene (H9268-5G, Sigma), potassium chloride (P217-500, FisherSci), puromycin (ant-pr-1, Invivogen), sodium chloride (6438, FisherSci), sodium deoxycholate (97062-028, VWR), sodium dodecyl sulfate (SDS) (74255-250G, Sigma), sodium fluoride (S6776-100G, Sigma), sodium orthovanadate (450243- 10G, Sigma), sodium pyrophosphate decahydrate (221368-100G, Sigma), Taq DNA ligase (M0208L, NEB), TERGITOL Type NP-40 (NP40S-100ML, Sigma), Tris base (T1378-5KG, Sigma), Triton X-100 (T9284-500ML, Sigma), tryptone (DF0123-17-3, FisherSci), Tween-20 (BP337-500, FisherSci), T5 exonuclease (M0363S, NEB), yeast extract (BP1422-2, FisherSci), and zeocin (ant-zn-1, Invivogen).



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    lukas kapitein  (Addgene inc)
    93
    Addgene inc lukas kapitein
    Lukas Kapitein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lukas+kapitein/pE4c+(Plasmid+%2317464)/bio_rxiv__2025__08__05__668525-205-18-20
    Average 93 stars, based on 1 article reviews
    lukas kapitein - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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